High-Throughput in Vitro Physiology for the Human Intestinal Epithelium

Our project entails the injection of various cargos into organoids formed by gastrointestinal stem cells in cultured and downstream analysis of the injected cargo. The 3D printed objects allowed us to mount the injection equipment with the confines of a physiologic chamber mounted on a automated microscope. The blue control-arm connects the microneedle holder to a robotic manipulator, allowing for fine control of a microneedle that is driven into the organoids. The white stage insert holds the culturing device containing the organoids in a specific orientation suitable for injection. A) 3D Printed Control Arm and B) Stage Insert in a Microinjection Platform
-Scott Magness, Magness Lab, Department of Cell Biology and Physiology